Verification of the Automated ELISA Assay for Hepcidin-25 in Human Serum

Autor(i)

  • Tara Rolić Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia Autor https://orcid.org/0000-0003-1731-6645
  • Sanja Mandić Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia Autor https://orcid.org/0000-0003-3921-8181
  • Iva Lukić Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia Autor
  • Vesna Horvat Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia; Medical Biochemistry Laboratory, Health Center Osijek-Baranja County, Osijek, Croatia Autor https://orcid.org/0000-0001-9554-4278
  • Ines Banjari Department of Food and Nutrition Research, Faculty of Food Technology Osijek, Osijek, Croatia Autor

DOI:

https://doi.org/10.26332/seemedj.v7i1.266

Ključne riječi:

enzyme-linked immunosorbent assay, hepcidins, homeostasis, iron

Sažetak

Introduction: Hepcidin-25, the bioactive form of hepcidin, is the master protein in regulating iron homeostasis. Serum concentrations, measured by different methods, are often incomparable and complicate results interpretation.

Materials and Methods: The aim was to verify the first fully automated enzyme-linked immunosorbent assay (ELISA) method, using the DRG Hybrid XL analyzer (DRG Instruments, Marburg, Germany) standardized against the mass spectrometry method. Intra- (CVi) and inter-assay (CVg) precision and bias were performed using commercially available controls with low (C1) and high (C2) concentrations. The reference interval was verified by analyzing serum samples of 20 healthy males.

Results: CVi = 9.1% (C1), 4.5% (C2); CVg = 8.9% (C1), 5.6% (C2); calculated bias was 33% for C1 and 20% for C2, respectively.

Conclusion: Verification of the fully automated ELISA method for hepcidin-25 in serum on the DRG Hybrid XL analyzer met the analytical acceptance criteria.

Preuzimanja

Objavljeno

2023-04-30

Broj časopisa

Svezak 7 Br. 1 (2023)

Rubrika

Articles

Licenca

Autorska prava (c) 2025 Southeastern European Medical Journal

Creative Commons License

This work is licensed under a Kreativni Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.